Coding

Part:BBa_K806002:Design

Designed by: Chang Ye   Group: iGEM12_SYSU-China   (2012-09-24)


hda - regulator of DnaA that prevents premature reinitiation of DNA replication


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 330
    Illegal BglII site found at 225
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 330
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Add restriction enzyme site on both end 2. clone the gene from genomic DNA extraction for better effect.


Source

we clone the gene in BL21 strain of E.coli, by using primer hda-F and hda-R hda-F sequence: GCTCTAGAGCCTGAACACACCGGCACAGCTCTCT hda-R sequence: GGACTAGTCCCTACAACTTCAGAATTTCTTTCACAAACGGA hda genebank id: AM946981.2

References